In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

The body hairs of the Saharan silver ant cause total internal reflection of light to make the ants almost ten times more reflective, preventing overheating and yielding their silver sheen, according ...

Refraction close refractionProcess by which a wave changes speed and sometimes direction upon entering a denser or less dense medium, eg a light ray changes direction when refracted by a lens. from a ...

Cool operator: the Saharan silver ant Cataglyphis bombycina. (Courtesy: P Landmann) A species of desert-dwelling ant is able to tolerate temperatures exceeding 50 °C because its body hairs act like ...

Science presenter Jon Chase explains total internal reflection. He demonstrates how total internal reflection can make a coin disappear inside a glass of water and bend a laser beam. The second ...

If you want to visualize what happens at a biological interface or on the surface of a cell, it is vital to eliminate the background that comprises all the stuff that sits beyond that first layer.

Bound states in the continuum (BICs) are a special class of resonant states. Although they have the same momentum and energy as the propagating modes in free space, yet they are not coupled to the ...

When we first saw "interactive touch-screen display" in the tips jar, it made us think of playing MegaTouch 9000 at a seedy bar and we were about to toss it in the ol' recycling bin. Then we decided ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...

The Wolfson Bioimaging Facility provides total internal reflection fluorescence microscopy (TIRF) with its multi-laser, dual-camera Olympus/Abbelight SAFe360 system which also offers single molecule ...