In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Total internal reflection microscopy is a light microscopy technique that relies on total internal reflection of illumination light to generate an evanescent wave that extends less than 200 nm from ...

Total internal reflection–fluorescence correlation spectroscopy (TIR-FCS) is an emerging technique that is used to measure events at or near an interface, including local fluorophore concentrations, ...

“There is no branch of detective science that is so important and so much neglected as the art of tracing footsteps,” says the fictional detective Sherlock Holmes. Now, UK researchers have developed a ...

Bound states in the continuum (BICs) are a special class of resonant states. Although they have the same momentum and energy as the propagating modes in free space, yet they are not coupled to the ...

The body hairs of the Saharan silver ant cause total internal reflection of light to make the ants almost ten times more reflective, preventing overheating and yielding their silver sheen, according ...

Refraction close refractionProcess by which a wave changes speed and sometimes direction upon entering a denser or less dense medium, eg a light ray changes direction when refracted by a lens. from a ...

ICSE and ISC 2026 board exams dates are out by CISCE. Important physics questions for Class 10 are also shared along with the ...

Cool operator: the Saharan silver ant Cataglyphis bombycina. (Courtesy: P Landmann) A species of desert-dwelling ant is able to tolerate temperatures exceeding 50 °C because its body hairs act like ...

When we first saw "interactive touch-screen display" in the tips jar, it made us think of playing MegaTouch 9000 at a seedy bar and we were about to toss it in the ol' recycling bin. Then we decided ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...